![]() ![]() In this stage the phosphorylation of fructose 6-phosphate to fructose-1,6-biphosphate takes place. The next step of glycolysis is also a rate limiting one. Hexokinase Activity Assay Kit (Colorimetric) To alter metabolism at this stage or quantify expression levels see the recommended products below. Out of the four types of the HK enzyme, HK2 is the most abundant, and is bound to the mitochondrial outer membrane. This stage is crucial for glycolysis as not only is it a rate limiting step, it’s high affinity for glucose means glycolysis can take place when serum levels of glucose are low. Once glucose has entered the cell it is phosphorylated by hexokinase (HK) to glucose-6-phosphate, and the process of glycolysis is initiated. Antibody Anti-Glucose Transporter GLUT1 antibody ![]()
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